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	Provedor de dados Ciência Rural (1) Genet. Mol. Biol. (1) Autor Manfroi,Ernandes (2) Didoné,Dielli Aparecida (1) Grando,Magali F. (1) Grando,Magali Ferrari (1) Lau,Elene Yamazaki (1) Morás,Andréa (1) Roesler,Eduardo A. (1) Roesler,Eduardo André (1) Yamazaki-Lau,Elene (1) Mais... Palavra-chave Co-cultivation conditions (1) GUS (1) Growth regulators (1) Protocol establishment (1) T-DNA delivery (1) Tissue culture (1) Triticum aestivum (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2015 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens Genet. Mol. Biol. Manfroi,Ernandes; Yamazaki-Lau,Elene; Grando,Magali F.; Roesler,Eduardo A.. Abstract Low transformation efficiency is one of the main limiting factors in the establishment of genetic transformation of wheat via Agrobacterium tumefaciens. To determine more favorable conditions for T-DNA delivery and explant regeneration after infection, this study investigated combinations of acetosyringone concentration and pH variation in the inoculation and co-cultivation media and co-culture temperatures using immature embryos from two Brazilian genotypes (BR 18 Terena and PF 020037). Based on transient expression of uidA, the most favorable conditions for T-DNA delivery were culture media with pH 5.0 and 5.4 combined with co-culture temperatures of 22 °C and 25 °C, and a 400 μM acetosyringone supplement. These conditions resulted in blue foci... Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-cultivation conditions; Protocol establishment; GUS; T-DNA delivery. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400470 Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes Ciência Rural Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki. ABSTRACT: The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402 Registros recuperados: 2 Primeira ... 1 ... Última

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